An intensive, five-day practical skills training on advanced techniques in fish health used for the detection and diagnosis of bacterial pathogens. Students will learn how to properly setup a bacteriology lab, the various types of culture media (including when and why they are used), bacteriology techniques plus differences between bacteria screening and diagnosis, including quality assurance and quality control applications. Common diagnostic techniques (e.g., streak plate for colony isolation, Gram’s stain, oxidative-fermentative test, antibiotic sensitivity tests) will be practiced on live bacterial cultures
•Perform standard screening and diagnostic techniques to detect bacterial pathogens of fish.
•Describe the importance of aseptic technique in a microbiology laboratory.
•Be able to confidently use different types of culture media and basic microbiology techniques.
•Define the terms: inoculation, differential vs. selective vs. enrichment media
•Be able to effectively isolate bacteria cultures and correctly perform a Gram's stain.
•Define the terms: Gram negative, Gram positive, differential stain, counter stain, bacillus, coccus, spirilla.
•Describe the applications of using antibiotic sensitivity discs and conduct an antibiotic sensitivity test on live bacteria.
•Define the terms: zone of inhibition, resistance, sensitivity, bacteriostatic, bacteriocide
•Define the terms: presumptive test, confirmatory test, oxidative, fermentative.
•Demonstrate how to use a biochemical test kit (API-20E) to identify Gram-negative bacteria.
•Define the terms: API 20E, cupule, McFarland turbidity standards.
Prerequisites: Level 2: Practical Fish Health for Aquaculture & Fisheries Ecology
For more information and registration, see course page here.